ABSTRACT
<p><b>OBJECTIVE</b>To observe the osteoinductive activity of demineralized bone matrix (DBM) and deproteinized bone (DPB) made from human avascular necrotic femoral head.</p><p><b>METHODS</b>The femoral head was cut into pieces with the size of 3 mm x 3 mm x 5 mm, which were made into DBM and DPB. These two kinds of biomaterials were cocultured with human bone mesenchymal stem cells (hBMSCs). Monolayer cells without biomaterials were cultured as control. Proliferative activity of hBMSCs was evaluated on days 1, 3, 5, 7 and 14. The concentration of alkaline phosphatase (ALP), osteocalcin (OC), and Ca(2+) were detected on days 1, 7, 14 and 21.</p><p><b>RESULTS</b>Cells cultured in DBM showed higher proliferative activity than did in DPB and monolayer cells (F =39.773, P <0.01). DBM and DPB also had osteoinductive activity. The concentrations of ALP (F=93.162, P <0.01), OC (F =236.852, P < 0.01), Ca(2+)(F =80.711, P <0.01) of DBM group were significantly higher than that of DPB and control groups.</p><p><b>CONCLUSIONS</b>In vitro, DBM and DPB made from avascular necrotic femoral head have osteoinductive activity when cocultured with hBMSCs, and the former is stronger than the latter.</p>
Subject(s)
Humans , Alkaline Phosphatase , Biocompatible Materials , Bone Demineralization Technique , Bone Matrix , Cell Biology , Bone and Bones , Cell Biology , Calcium , Cell Proliferation , Cells, Cultured , Femur Head Necrosis , Mesenchymal Stem Cells , Cell Biology , Osteocalcin , OsteogenesisABSTRACT
Cerebrovascular reserve is an important parameter for evaluating cerebrovascular disease,and it has great clinical significance.There are a number of determination methods for cerebrovascular reserve,such as position emission tomography(PET),single photon emission computed tomography(SPECT),Xenon-133 inhalation technique,Xenon CT,perfusion CT, magnetic resonance imaging(MRI),transcranial Doppler(TCD),transcranial harmonic perfusion imaging(HPI),near infrared spectroscopy(NIR),and Laser Doppler vibrometry(LDV), however,there have been no unified criteria up to now.The provocation test that makes cerebral blood flow increase includes CO_2 inhalation test,breath holding test,and acetazolamide test,etc. The article focuses the discussion on the advantages and disadvantages of the above determination methods.